An assembly of reads, contigs and scaffolds

A blog on all things newbler and beyond

Archive for July, 2011

Newbler output V: the 454ContigScaffolds.txt and 454ScaffoldContigs.fna

Posted by lexnederbragt on July 12, 2011

Filling the gaps (picture from http://www.ifaonline.co.uk)

In the post on what is new in newbler version 2.6, I introduced the -scaffold option. Briefly, with this option instances (i.e. the consensus sequence) of repeats are placed in gaps. As I mentioned, setting -scaffold results in two extra files. With this post, I will explain these in detail.

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Posted in How it works, Newbler output | Tagged: , , , , , , | 1 Comment »

What is new in newbler 2.6

Posted by lexnederbragt on July 12, 2011

The latest version of newbler, version 2.6, has some welcome additions for input and output. As I have so far only treated de novo assembly, I will skip the updates on the gsMapper (except for mentioning that it is now able to provide a bam file using the -bam option).

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Posted in Using newbler | Tagged: , , , | 21 Comments »